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Recently, Comatose (CTS), a plant homologue of peoples ABCD1, has been shown to possess acyl-CoA thioesterase (ACOT) activity, which is recommended that this activity is necessary for transport of acyl-CoA into peroxisomes. Nonetheless, the complete transportation method is unidentified. Right here, we expressed individual His-tagged ABCD1 in methylotrophic fungus, and characterized its ACOT activity and transportation mechanism. The expressed ABCD1 possessed both AT