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Currently no research indicates that either pathogenic and non-pathogenic V. parahaemolyticus possess development heterogeneity in a person environment, such in gastric and abdominal liquids. The tlh gene is present both in pathogenic and non-pathogenic V. parahaemolyticus strains, as the tdh and trh genes are merely contained in pathogenic strains. This study firstly applied simulated human being gastric fluids to explore development variability of 50 strains of V. p