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Percent intensity contributed by each amino acid was determined and converted into weights to (1) update the previously published C-score, and (2) con-struct a native Fragmentation Propensity Score (nFPS). Both scoring systems showed an improvement in protein identifica-tion or characterization in comparison to traditional methods, and overall increased confidence in results with fewer matched fragment ions but with high probability nTDMS fragmentation patterns. Given the rise of nTDMS as a tool for struc-tural mass spectrometry, we forward
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