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The findings revealed that the acclimatized sludge two-stage AS system could be deployed to effectively treat landfill leachate with moderate concentrations of compounds and trace organic contaminants. The acclimatized sludge AS is an efficient wastewater treatment solution for developing countries with limited technological and financial resources.Domestic and industrial wastewater treatment systems are vital in the protection of natural ecosystems and human health. Identification of microbial communities in the systems is essential to

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