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These results suggested that gut microbiome altered by TBT exposure was involved in the TBT-induced increased body weight, impaired glucose and insulin homeostasis and endocrine disruption in mice, providing significant evidence and a novel perspective for better understanding the mechanism by which TBT induces toxicity.Technological progress and the development of laboratory techniques and bioinformatics tools have led to the availability of ever-increasing amounts of biological data including genomic, proteomic, and transcriptomic seq