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To investigate the effects of a 9.3-μm carbon dioxide (CO ) laser and silver diamine fluoride (SDF) on the prevention of enamel demineralisation and inhibition of cariogenic bacteria. Enamel blocks were applied with Laser (Group-1), SDF (Group-2), Laser + SDF (Group-3) and no treatment (Group-4), and then subjected to an 8-day pH-cycling for cariogenic challenge. Lesion depth and cross-sectional micro-hardness were assessed. Surface morphological and chemical changes were studied using scanning electron microscope (SEM) with energy disper