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Given the branched-chain structure and high positive charge density of St-AD, it had a higher efficiency in treating real wastewater and organic-phosphorus-simulated wastewater than PAM and linear cationic St-CTA. These results may serve as references for the design and selection of a suitable flocculant in treating target wastewaters.Chitinases play crucial roles in enzymatic conversion of chitin and biocontrol of phytopathogenic fungi. Herein, a chitinase of glycoside hydrolase (GH) family 19, SaChiB, was cloned from Streptomyces alfa