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To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. 128 three-week-old weaned rats underwent high-fat diet and exercise (60-70% VO , 1h/day, 5days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting. (1) The protein of KISS-1 and GPR54 inc
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