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Agarose gel electrophoresis (AGE) and dynamic light scattering (DLS) showed that the binding ratio was roughly 21 (HSA/QDs), resembling sandwich-like structures. Furthermore, the secondary structure of HSA depended on the concentration of added QDs and the incubation time. The results preliminarily uncovered the physicochemical properties of QDs in the presence of proteins and elucidated the role of time evolution. These will inspire us to make the fluorescent QDs more biocompatible and use them in a proper way.l-ascorbic acid (AA) or

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