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This research aimed to describe the rates and molecular mechanisms of mupirocin weight in medical Staphylococcus aureus isolates from Tygerberg Hospital, and to describe its connection with stress types. TECHNIQUES We retrospectively selected 212 S. aureus isolates which were identified from blood samples and pus swabs throughout the years 2009-2011 and 2015-2017. The isolates were identified making use of conventional microbiological methods and genotyping was done using spa t