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Five biomarkers, such as Eubacterium_1 coprostanoligenes, Clostridiales, Phascolarctobacterium, Acidaminococcaceae and Ruminococcaceae_UCG_002 were selected by Lefse analysis. More favorably correlated bacteria had been Terrisporobacter and Oscillospiraceae (r = 0.7 and the highly unfavorable correlation was found between Ruminococcaceae_NK4A214_group and [Ruminococcus]_gauvreauii_group (roentgen = 0.64). Therefore, hydrogen sulphide ruined the microbial stability and enhanced the ab