https://ml210agonist.com/prope....r-diagnosis-of-bushy
For this, the DAD V1M mutant (bDAD) was overexpressed, purified, and reconstituted with various material ions. Kinetic assays assessing the activity of this reconstituted chemical as well as the substrate- and product-dependences regarding the reaction kinetics were performed. The outcome from reconstitution associated with apoprotein with a variety of metal ions offer the need for an Fe3+ center for enzyme task. Reaction rates revealed easy saturation kinetics for DHA with values for kcat and KDHA of 2.4
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