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Therefore, the aim of this research was to clarify this relationship. In vitro, we stimulated chicken macrophage cells (HD11) with lipopolysaccharide, then examined the correlation between SPOP and IL1β or IL8 appearance making use of quantitative real time polymerase string effect (qRT-PCR). In vivo, we infected 7-days-old chickens with Salmonella Typhimurium, then examined the relationship between SPOP appearance therefore the resistant response, including IL1β and IL8 expression, IgA manufacturing, and microbial lot