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The collection of experimental information parameterized the computational biophysical method. Through molecular docking, the binding web site micro-environment was revealed is composed mainly by non-polar amino acids. Furthermore, molecular dynamics, along with umbrella sampling, have been in agreement with the thermodynamic variables acquired by fluorescence assays and showed that big protein moves aren't contained in IL-1β, corroborating the circular dichroism data.Severe severe respira
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