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), pumpkin (Cucurbita moschata Duch.), and winter squash (Cucurbita maxima Duch.).Doubled haploid (DH) technology is very advantageous in plant breeding. This technique is beneficial for reducing the time required to obtain pure lines and contributes to the selection efficiency. Using this technique, 100% homozygosity can be achieved in a single generation, while the development of stable lines using the traditional self-pollination method takes from 6 to 8 years. It has long been used in diverse crops including cucurbits. DHs can be obtained via

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