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From aerial parts of Stevia lucida Lagasca was isolated the natural mixture of isomeric eudesmanolides helenin. The identification and quantification of the constituents of helenin (alantolactone 1 and isoalantolactone 2, ratio 37) was performed through the quantitative analysis of its 1D and 2D NMR spectra.Recent clinical trial data showed that injectable long-acting antiretroviral treatment (LA-ART) every four or eight weeks could become an alternative option for HIV treatment or prevention. The purpose of our study was to explore perc