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Generalized linear mixed models were used to determine effects of BM, SVL, and BC on the poison toxicity. We recorded significant changes in the insect heart contractility after treatment with the whole poison and separated fractions. We found an individual variation in cardiotoxicity of the parotoid extract which was explained by the body size of toad. Poison of smaller toads displayed a negative, whereas poison of larger toads positive, chronotropic effect on the heart contractility. Thus, we conclude that the effectiveness of parotoid secr