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The outcomes indicated that the sulfonation ability regarding the three reagents was in the order of ClSO3H H2SO4 TsOH, while hydrothermal therapy offered the highest complete acidity, and biggest level of acid groups (e.g., SO3H, COOH and Ar-OH). Biochar X with higher O/C and N contents, and less graphitic functions revealed superior acidic properties than biochar H under all the used treatments. The hydrolytic efficiencies associated with the sulfonated biochar