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We conclude that the supplementation with probiotic algae-associated bacteria can significantly enhance biomass and EPA production of N. oceanica.This work studied the hydrothermal liquefaction of Chlorella pyrenoidosa and effect of emulsification on upgrading the bio-oil. The fuel properties and storage stability characteristics of emulsion fuels were explored. The combustion characteristic analysis showed that the ignition temperatures of emulsion fuels (139.6-151.3 °C) were lower than that of bio-oil (176.9 °C). Besides, emulsion fu