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Background Vaginal agenesis is a rare condition with different surgical technique to achieve the purpose of neovaginal reconstruction. The main difference of various approach lies in the graft material used in covering the newly-formed cavity. Objectives The purpose of this retrospective study was to describe the surgical procedure and outcomes of autologous buccal mucosal grafting in the reconstruction of neovaginal. Methods Sixteen patients with vaginal agenesis admitted in our department between January 2016 and January 2019 were inclu