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This work provided an economical and eco-friendly preparation of recombinant chitosanase to scale up the hydrolysis of chitosan towards tailored oligosaccharides in the near future.The possibilities of applying the pure Lagrangian vortex methods of computational fluid dynamics to viscous incompressible flow simulations are considered in relation to various problem formulations. The modification of vortex methods-the Viscous Vortex Domain method-is used which is implemented in the VM2D code developed by the authors. Problems of flow si