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Results Our experiments confirmed how using appropriate image pre-processing in the right order can improve the performance of deep neural networks in terms of better classification and segmentation. Conclusions This work investigates the appropriate pre-processing steps for CT and MR images of prostate cancer patients, supported by several experiments that can be useful for educating those new to the field (https//github.com/NIH-MIP/Radiology_Image_Preprocessing_for_Deep_Learning).Surfactant protein B (SP deficiency is a severe monog