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The impact of acute and chronic exposure of nonylphenol (NP) on behaviour, histopathology, haematology and biochemical parameters of Labeo rohita (Hamilton, 1822) was investigated in the current study. The 96 h LC50 of NP for L. rohita was estimated to be 0.548 mg L-1. Acute toxicity of NP induced several behavioural alternations. Further, sub-lethal NP exposure for a period of sixty days to 1/10th (0.0548 mg L-1), 1/15th (0.0365 mg L-1) and 1/20th (0.0274 mg L-1) of 96 h LC50 resulted a reduction in total erythrocyte count and haemoglo