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The expression levels of PSMG3‑AS1 and miR-143-3p were closely and inversely correlated with each other. High expression levels of PSMG3‑AS1 predicted poor survival. In HCC cells, overexpression of PSMG3-AS1 led to increased proliferation rates. Overexpression of miR-143-3p played an opposite role and reversed the effect of overexpression of PSMG3‑AS1. miR-143-3p may target PSMG3‑AS1 to inhibit the proliferation of HCC cells. miR-143-3p may target PSMG3‑AS1 to inhibit the proliferation of HCC cells. This study tried to evaluate whether