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61±81.02 and 254.50±73.29 (P=0.47), and those for high quality and poor quality embryos were 266.02±98.56 and 295.71±70.64 (P=0.99), respectively. There was no significant difference in cumulus cell mtDNA copy number between implanted and non-implanted embryos or between high quality and poor quality embryos. The mitochondrial membrane potential, ROS levels and ATP content of the corresponding cumulus cells did not differ significantly between high quality and poor quality groups. Measurement of cumulus cell mtDNA copy number might not p