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In the current study, the trehalose (TRE) effects as an additive regarding the architectural security and function of uricase had been investigated. For recombinant appearance of UOX in E. coli BL21 cells, firstly the coding series was subcloned to the pET-28a vector and after induction with IPTG, the recombinant UOX had been purified by affinity chromatography making use of a Ni-NTA agarose column. To specify the trehalose effects from the urate oxidase (UOX) framework, optimum pH, optimum temperature,
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