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Despite the benefits of nano-Ag, more and more study indicates that it could have prospective biotoxic results. Research indicates that people who consume nanoparticles by lips have the best uptake within the intestinal tract, and that the colon location is one of susceptible to harm and results in the illness. In this study, we examined the harmful aftereffects of various concentrations of Ag-NPs on normal peoples colon cells (NCM46 and personal cancer of