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Our outcomes suggest that this technique favours the increasing loss of adjacent hydrogen atoms.Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the recognition of isomiRs with double specificity and magnification. The solitary nucleotide variant of isomiRs in terminals and/or internal series might be discriminated by this tactic. That way, let-7a isomiRs in MCF-7 and MCF-7R mobile lines had been analyzed.Correction for 'Substrate and product binding inside a sti