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Heavy metals have caused widespread concern due to their adverse effects on aquatic organisms. However, there are few studies on their tolerance mechanism. In this study, the tolerance mechanisms of Cyclotella sp. to Cr(VI) were explored. The increase of antioxidant enzymes activity acting as a defense mechanism could help Cyclotella sp. to reduce the oxidative damage caused by the heavy metal Cr(VI). Cr(VI) was also combined with the functional groups on the cell surface to detoxify and was transported into the cell by binding to the carr