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The present study investigated the ecotoxicity of raw mining effluent from the largest molybdenum (Mo) open-pit mine in the Qinling mountains, China, and the treated effluent with neutralization and coagulation/adsorption processes, using zebrafish (Danio rerio). The results showed the following (1) the mining effluent is acid mine drainage (AMD) and is highly toxic to zebrafish with a 96-h median lethal concentration (LC5 of 3.80% (volume percentage) of the raw effluent; (2) sublethal concentrations of the raw effluent (1/50, 1/10,