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No toxicity was found to all organs of mice. The study developed an efficient way to enhance the antibacterial activity of CS dressing loaded AgNPs at low toxicity, which has great potential in wound care application.Aqueous two-phase system (ATPS) is a technique used for the separation of biopolymers in two aqueous phases. Some combinations of biopolymers can form a water-in-water (W/W) emulsion due to steric exclusion and thermodynamic incompatibility between these biopolymers under some specific conditions. In this work, the formation