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The microwells are manufactured from agarose, a nontoxic material suited to cell growth and spheroid development. HepG2 cells loaded into customizable microwells formed spheroids through agarose-assisted aggregation within one or two days. In addition, the agarose matrix permits the same system to be utilized in DNA damage evaluation. Particularly, the comet assay enables quantification of DNA breaks on the basis of the increased migration of wrecked DNA through agarose during el