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Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have reduced SY penalization when cropped in warm-dry conditions, which constitute the best target environments for this technology. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.BACKGROUND SRY is one of the important genes involved in the process of human sex determination. The disturbed sex determination caused by SRY mutation accounts for 10-15% of cases with 46, XY sex reversal. Recent