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As it is a neglected exotic disease, the rewards for the growth of brand new drugs tend to be insufficient. It is critical to know Leishmania virulence factors that add most towards the illness so that you can develop drugs. In our work, we have produced L. infantum prolyl oligopeptidase (rPOPLi) in Escherichia coli, and investigated its biochemical properties as well as the effect of POP inhibitors on its enzymatic activity as well as on the inhibition regarding the mac