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01), but an increased p-Akt/Akt ratio (P less then 0.05) and an elevated level of p-mTOR phosphorylation (P less then 0.05). CONCLUSIONS Triptolide can decrease the activity and induce the apoptosis of TM3 Leydig cells, and meanwhile inhibit the AMPK and activate the Akt/mTOR pathway.Objective To identify differentially expressed proteins in the sperm of oligoasthenozoospermia (OAZ) patients and provide some theoretical evidence for the study of OAZ. METHODS We collected semen samples from 30 OAZ patients and another 30 normal healthy male