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These studies conducted analyses of both the whole and parts of the brain in terms of neuronal activity and blood perfusion, but the procedures were laborious and time-consuming. We have developed a convenient and time-saving software package using MATLAB 2014a to automate the data preparation and analysis of across-voxel coupling between multimodal images. We have developed a convenient and time-saving software package using MATLAB 2014a to automate the data preparation and analysis of across-voxel coupling between multimodal images.Diffusion MRI is a non-