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PURPOSE To assess the diagnostic performance and generalizability of logistic regression in classifying primary vitreoretinal lymphoma (PVRL) versus uveitis from intraocular cytokine levels in a single-center retrospective cohort, comparing a logistic regression model and previously published Interleukin Score for Intraocular Lymphoma Diagnosis (ISOLD) scores against the interleukin 10 (IL-1-to-interleukin 6 (IL-6) ratio. DESIGN Retrospective cohort study. PARTICIPANTS Patient histories, pathology reports, and intraocular cytokine level