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2-17.2% compared with those in the suspension. The genera Ercella and Petrimonas along with the methanogenic archaea Methanosaeta and Methanosarcina on the carbon cloth may facilitate direct interspecies electron transfer, thereby enhancing methane production.Biochar has been proven as a soil amendment to improve soil environment. However, mechanistic understanding of biochar on soil physical properties and microbial community remains unclear. In this study, a wood biochar (W, was incorporated into a highly weathered tropical soil, and a