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The prevalence of cholelithiasis and gallbladder cancer may be different across ethnic groups. To study the prevalence of cholelithiasis and gallbladder cancer among Aymara individuals. An abdominal ultrasound was carried out in a sample of 182 Aymara women aged 46 ± 16 years and 76 Aymara men aged 55 ± 16 years. In addition, the histopathological reports of both patients with a history of previous cholecystectomy and those operated after the study were reviewed. Ultrasound was normal in 150 participants (58%), 76 had cholelithiasis (30%) and 32 (12%) had a