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Right here, we present the development and application of a lab-on-a-chip spray approach that integrates quick test preparation, mixing, and deposition to integrate with a variety of nanoanalytical practices in biochemistry and biology, providing improved spectroscopic sensitiveness and single-molecule spatial resolution. We display that this process makes it possible for multidimensional study of heterogeneous biomolecular systems over several size scales by nanoscopy and vibrational spectroscopy