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Complete mydriasis was observed in 5/10 birds (mean weight 97.4g). Pupil size at t and t did not differ from baseline (P.05) in treated eyes. No adverse effects were seen during the study period. Single-dose topical rocuronium bromide (0.15mg) is a safe and effective medium duration mydriatic agent in scops owls. Further studies are needed to evaluate bilateral topical application and standardize the mydriatic protocol. Single-dose topical rocuronium bromide (0.15 mg) is a safe and effective medium duration mydriatic agent in scops