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obliquus were significantly up-regulated in the presence of nTiO2. The arginine and proline metabolic pathways related to the anti-stress effect of algae cells also showed positive stimulation. The results of this study provide an important reference and a research basis for in-depth understanding of the environmental effects of MNMs and co-existing contaminants.Sediment samples were collected from nine sites of three sections, in the intertidal zone of Haizhou bay in July 2018. The abundance and characteristics of microplastics in the