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We determined the Hb threshold with all the highest connected sensitivity and specificity for finding ID among South African ladies residing at 1700 m above sea level. In a cross-sectional study of 492 18-25-year-old females, we sized Hb and metal standing biomarkers. Using receiver working characteristic curves, we determined the Hb threshold with optimum Youden Index for detecting ID. This limit of less then 12.35 g/dL resulted in a 37.2% anaemia prevalence