https://www.selleckchem.com/pr....oducts/4-chloro-dl-p
Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant library in traditional TIS, the defined mutant library sequencing (DML-Seq) has advantages as 1) efficient mutagenesis; 2) low bottleneck effects; 3) avoid hotpots caused by scree