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In summary, this study demonstrates the feasibility and benefits of 3D engineered tissue environment combined with perfusion flow in vitro for culturing stem cell-derived IPCs.It is commonly assumed that cerebral asymmetry is unidimensional, but evidence increasingly suggests that different brain circuits are independently lateralized. This might explain why the search for a laterality gene has provided multiple candidates, each with weak linkage. An alternative possibility is that there is a single genetically invariant source of later