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Cladosporium and Penicillium isolated from the samples were related to the estrus stage, and yeast was grown in diestrus samples. The 16srRNA gene existed in all of the estrous uterine samples in which the bacterial culture was negative. However, the presence of this gene was proven in two samples (33.30%) of negative bacterial culture samples from the diestrus and anestrus stages. In conclusion, the normal bitches' uteri were infected with various bacteria in estrus, diestrus and anestrus stages of the estrous cycle, and it could coinci