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Trypanosoma cruzi, the causative agent of Chagas disease, can be transmitted to the offspring of infected women, which constitutes an epidemiologically significant parasite transmission route in non-endemic areas. It is relevant to evaluate differentially expressed factors in T. cruzi-infected pregnant women as potential markers of Chagas congenital transmission. Circulating levels of twelve cytokines and chemokines were measured by ELISA or cytometric bead array in T. cruzi-infected and uninfected pregnant women in their second trimest