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Congenital cardiovascular disease (CHD) is among the most prevalent beginning defects which causes baby death around the world. The timely and successful surgical treatment of CHD on newborns after delivery requires accurate detection and trustworthy diagnosis during pregnancy. Nonetheless, there are no biomarkers that may act as an early diagnostic factor for CHD patients. tRNA-derived fragments (tRFs) being reported to relax and play a crucial role within the occurrence and development of numerou