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Finally, the review highlights the existing challenges and perspective solutions which could be promising for future in vivo applications.Raman-encoded gold nanoparticles (NPs) have been widely employed as photostable multifunctional probes for sensing, bioimaging, multiplex diagnostics, and surface-enhanced Raman scattering (SERS)-guided tumor therapy. We report a strategy toward obtaining a particularly large library of Au nanocapsules encoded with Raman codes defined by the combination of different thiol-free Raman reporters, encapsula