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The aim of this study was to elucidate whether ovarian tissue is able to withstand a double freezing-thawing procedure. Human ovarian cortical biopsies from 4 thawed whole ovaries were divided into 4 experimental subgroups (a) frozen-thawed non-grafted group, (b) frozen-thawed xenografted group, (c) refrozen-rethawed non-grafted group, and (d) refrozen-rethawed xenografted group. Xenografting was performed using 8 severe combined immunodeficient mice for a total duration of 21days. The following analyses were conducted classic hematoxyli
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